Valorization of biodiesel side stream waste glycerol for rhamnolipids production by Pseudomonas aeruginosa RS6

Biodiesel side stream waste glycerol was identified as a cheap carbon source for rhamnolipids (RLs) production which at the same time could improve management of waste. The present study aimed to produce RLs by using Pseudomonas aeruginosa RS6 utilizing substrate and evaluate their physico-chemicals properties. Fermentation conditions such temperature, initial medium pH, concentration, nitrogen sources concentrations resulted in different compositions mono- di-RLs produced. maximum 2.73 g/L obtained when P. grown basal salt supplemented with 1% 0.2 M sodium nitrate 35 °C pH 6.5. At optimal fermentation conditions, emulsification index (E24) values cooking oil, diesel benzene, olive petroleum, kerosene were all above E24=50%. surface tension reduction from 72.13 mN/m 29.4–30.4 better than activity some chemical-based surfactants. produced possessed antimicrobial activities against Gram-negative Gram-positive bacteria ranging 37% 77% growth inhibition 1 mg/mL used. Concentrations below 1500 ?g/mL did not induce phytotoxicity effects on tested seeds (Vigna radiata) compared chemical-based- surfactant, SDS. Furthermore, zebrafish (Danio rerio) embryos only exhibited low acute toxicity an LC50 value 72.97 48 h exposure suggesting green eco-biochemical worthy future applications replace